Services

SERVICES

Antibody-Drug Conjugate and Bioconjugation Services

antibody drug conjugates diagramTCRS has ample experience in providing antibody-drug conjugate related services to its clients. Currently, TCRS is engaged in supporting several biotech and pharmaceutical companies in the development of their ADC programs.

TCRS has a variety of highly potent warheads/payloads in stock for advancing its clients' ADC projects. A selection of cleavable and non-cleavable linkers and spacer molecules is also available.

TCRS provides the following services:

  • Preparation of ADCs, Antibody-Enzyme and Antibody-Liposome conjugates
  • Design and synthesis of potent cytotoxic compounds and payloads with linkers
  • Customized design and synthesis of generic linkers
  • Preparation and purification of bioconjugates from milligram- to gram- scale
  • Modification of carbohydrates, polysaccharides, and glycoconjugates
  • Preparation of fluorescent probes

TCRS conducts all work with highly toxic/cytotoxic compounds and payloads in its specially designed containment laboratory, which is accessible only to authorized and trained TCRS chemists. This laboratory has an independent air-handling (HVAC) system and is equipped with certified chemical fume hoods, HEPA-filtered ventilation and enclosed, HEPA-ventilated weighing stations. Compounds of high toxicity are handled in a glove box with a closed-loop containment (CLC) system that operates under negative pressure.

The purification of bioconjugate molecules and antibody-drug conjugate precursors is supported by the following instrumentation:

NMR instruments: • Bruker Avance 500-MHz NMR with Triple Inverted Resonance Probe (TXI), optimized for 1H observation, and LC capability
• Varian Inova 300-MHz NMR spectrometer with VNMRj software
LC-MS instruments: • Agilent 1100 LC-MSD trap
• Agilent 1200 LC-MSD

HPLC systems: • Analytical HPLC systems (e.g., Varian Prostar 210) with a host of analytical columns including chiral columns and PDR chiral detector
• Preparative HPLC systems (e.g., Varian Prepstar 218, Rainin Dynamax SD-1), equipped with fraction collectors (Varian Model 701); auto-sampler for high-throughput purification; Agilent diode array UV detector, Varian UV-Vis detector, PDR-Chiral Advanced Laser Polarimeter detector; preparative Chiralpak-IA, 5 cm ID × 50 cm L, 20 μ, chiral column, and other semi-prep/prep columns; Sephadex and size-exclusion columns.


Glove box with closed-loop containment systemProcess workdtation with top mount HEPA filtrationWorking on Antibody-Drug Conjugates


The Chemistry Research
Solution LLC


360 George Patterson Blvd.
Suite 108
Bristol, PA 19007

 

chemistry@tcrs-us.com


215-788-3603
800-699-9126
267-401-1028 (Fax)

 

Custom Synthesis
Antibody-Drug Conjugates
Medicinal Chemistry
Analytical Services
Protein NMR
Process Development
& Scale-up
GMP Manufacturing

 

 

 

Antibody-Drug ConjugatesDownload a  new brochure (1.1 MB).